Detection and analysis of Mycoplasma suis disease in a pig farm in Xinjiang region
Liu Shuhua
Li Chaoqun
Liang Fanxin
Chen Xin
Hou Mengzhe
Li Lianrui
Abstract：In order to understand the infection situation of Mycoplasma suis in a pig farm in Xinjiang region,200 samples of anticoagulant blood of piglets in a pig farm in Xinjiang region were collected.Blood pressure tablet,blood smear staining microscopy and common PCR method were used to detect Mycoplasma suis.16S rRNA gene primer of Mycoplasma suis was used,and blood DNA was used as template.After amplification,cloning,identification and sequencing,homology comparison and genetic evolution analysis were performed.The results showed that 58 positive samples of Mycoplasma suis were detected by blood pressure smear,blood smear and PCR.Using the 16S rRNA gene primer of Mycoplasma suis and blood DNA as the template,the gene of Mycoplasma suis was successfully cloned and named XJP1(has been included by Genbank,entry number:PP526316).Homology comparison and genetic evolution analysis showed that XJP1 was the same cladus with Mycoplasma suis(entry number:KC907396)and Mycoplasma suis(entry number:HQ259257),and the homology was 96.9％～99.6％.The study showed that the piglets in the farm were infected with Mycoplasma suis,and the test results will provide basic data for the prevention and control of Mycoplasma suis infection in the farm and the further study of the disease.
Keywords：Mycoplasma suis16S rRNA sequenceXinjiang region
Publication Date：2024-09-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )