Study on phylogenetic relationship of three sheep based on mitochondrial ATP6 gene
Wang Jingyao
Hai Chunhua
Zhang Junzhe
Li Zhengyu
Liu Xiaoye
Li You
Abstract：The experiment aimed to explore the phylogenetic relationships among Tibetan sheep,Tan sheep,and Small-tailed Han sheep in the Gansu Province,providing a reference for the protection and rational use of local sheep germplasm resources in Gansu Province.In the experiment,partial sequences of the mitochondrial ATP6 gene from Tibetan sheep,Tan sheep,and Small-tailed Han sheep were amplified and sequenced.The sequencing results were processed and compared with mitochondrial ATP6 gene sequences from various domestic and international sheep breeds.After the comparison,genetic diversity analysis was conducted using the DnaSP 5.0 software,and a phylogenetic tree was established using the MEGA-X software to explore the phylogenetic relationships between different sheep populations.The results showed that the number of haplotype selected for the Tibetan sheep population,Tan sheep population,and Small-tailed Han sheep population were 6,6,and 5,respectively,with haplotype diversities of 0.51579,0.92857,and 0.93333,and nucleotide diversities of 0.00293,0.00552,and 0.00520,respectively.The phylogenetic tree results indicated gene flow between the three sheep breeds and other domestic and international sheep breeds.The results showed that the Tibetan sheep,Tan sheep,and Small-tailed Han sheep had different degrees of kinship with the 21 other breeds of sheep,and attention should be paid to avoiding crossbreeding with other breeds when carrying out the breeding work of these three breeds of sheep.
Keywords：SheepATP6 geneGenetic diversityPhylogenetic evolution
Publication Date：2024-08-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )