Research progress on molecular detection methods of swine acute diarrhea syndrome coronavirus
Shen Qiuping
Huang Zihui
Sun Li
Sun Mengfan
Cai Heng
Zhuang Linlin
Abstract：SADS-CoV is an emerging porcine alphacoronavirus that causes severe vomiting,diarrhea,and dehydration in piglets under 5 days old,leading to high mortality rates.Early and accurate diagnosis is of great practical significance for the scientific prevention and control of SADS-CoV infection.This article reviews the research progress on molecular detection methods for SADS-CoV,including ELISA,RT-PCR and its derivative techniques(multiplex RT-PCR,real-time fluorescent quantitative RT-PCR,multiplex real-time fluorescent quantitative RT-PCR,droplet digital PCR),and nucleic acid isothermal amplification technologies(LAMP,RPA).The advantages and disadvantages of these detection methods are summarized and discussed,and the future development direction of SADS-CoV molecular detection methods is prospected,aiming to provide a reference for the scientific prevention and control of SADS-CoV.
Keywords：SADS-CoVELISART-PCRLAMPRPA
Publication Date：2024-07-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 70-74 )