Molecular biological identification and analysis of Thaleria annulata in a cattle farm in Kashgar
Huang Kaijun
He Bo
Li Fei
Xiong Na
Hu Jinglei
Shao Chengxiang
Wu Junyuan
Abstract：The purpose of the experiment was to identify the etiology of Simmental cattle in a cattle farm in Kashgar,and to detect the blood of cattle suspected to be infected with Thaleria by PCR.The 18S rRNA gene was amplified and sequenced by PCR,the phylogenetic tree was constructed by MEGA 7.0 software,and the genetic evolution was analyzed at the same time.The results showed that the sequence of 18S rRNA gene was 100.0％homologous to that of Theleria annulata isolated from Xinjiang,China(OM438135.1),indicated that the infected cattle were infected with Theleria annulata.Phylogenetic tree analysis showed that the strain was closely related to Chinese and Pakistani isolates.The study indicates that the pathogen causing the disease of Simmental cattle in a cattle farm in Kashgar is Thaleria annulata.
Keywords：KashgarThaleria annulataPCR18S rRNAIdentification and analysis
Publication Date：2024-02-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )