PCR program optimization and molecular identification of Actinobacillus pleuropneumoniae serotype 2
Li Haili
Lang Limin
Xu Yindi
Jiao Wenqiang
Zhu Wenhao
Zhang Lixian
Zhang Qingxian
You Yi
Hou Zihua
Yang Suxin
Xu Feng
Zheng Wanlu
Wang Keling
Abstract：Porcine contagious pleuropneumonia also known as necrotic pleuropneumonia, is a high-ly contagious and lethal respiratory infectious disease caused by Actinobacillus pleuropneumoniae. The main characteristic of this disease are acute hemorrhagic fibrinous pneumonia and chronic fi-brinous necrosis. Pigs at all ages and gender were susceptible to this disease and caused acute mortality. Actinobacillus pleuropneumoniae has 15 serotypes, there are no or weakly cross protec-tion between different serotypes, which brought great difficulties to control , diagnosis and im-mune prevention this disease. So it is particularly important to establish a rapid molecular identification method for serotype. In order to establish a rapid, simple method to identify Porcine Contagious pleuropneumoniae serotype , this research identified Actinobacillus pleuropneumoniae from swine lung and trachea with clinical of suspection , through serotype identification, determined type 2 serotype. In order to determine the accuraate serotype, the method of molecular identification was studied. 11 isolated strains were identified as serotype 2 Actinobacillus pleuropneumoniae. This provides a theoretical basis for molecular identification the infectious porcine Actinobacillus pleuropneumoniae serotype , and provides a simple method for the future clinical serum identification.
Keywords：Porcine contagious pleuropneumonia Actinobacillus pleuropneumoniaeMolecular iden-tificationSerotype 2
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 33-39 )