Amplification, Clone and Prokaryotic Expression of ORF2 Gene of Feline Calicivirus Isolated from Leopard
Liu Qiuyan
Ying Ying
Wang Yiming
Chen Xiaoqing
Zhao Yanli
Hu Guixue
Abstract：In order to prepare the antigen for serological detection of Feline calicivirus, the primers were designed in according to the gene sequence of 1 FCV isolated from a leopard preserved in our laboratory. The 1 734 nucleotides in open reading frame 2 (ORF2) were amplified and cloned into expression vector pET-28a to construct recombinant plasmid named pET-28a-FCV1 734. The recom-binant plasmid was transformed into E. coli. The expression product induced by IPTG was determined by SDS-PAGE and Western blot. The result proved that the expressed protein had good reactogenicity.
Keywords：Feline calicivirusORF2 geneExpression
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )