Rapid detection method preliminary establishment of infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification
Yang Zuofeng
Shi Lin
Deng Wenchao
Guo Yan
Dong Na
Wang Zhu
Zhao Pei
Zhang Xin
Zhang Yawei
Abstract：The objective of this study is to develop a rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of infectious bursal disease virus (IBDV). Six primers were designed to amplify the VP1 gene of IBDV using PrimerExplor-er V4. The optimal reaction condition of the current RT-LAMP for IBDV was 65 ℃ for 60 mins. It was capable of detecting IBDV from clinical samples and differentiating IBDV from other avain vi-ruses, and does not require an additional expensive equipment. The minimum detection limit of the RT-LAMP assay was 10-6, making this assay approximately 100-fold higher than that of one-step RT-PCR. The assay were evaluated by comparison with RT-PCR, by 93.8% consistence. The specificity and simplicity of the assay could make it a useful method for the detection of IBDV infection.
Keywords：IBDVLoop-mediated isothermal amplificationDiagnosis method
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 12-17 )