Detection method establishment for IBV by fluorescence quantitative PCR and the detection of IBV in infected chickens
Yu Changyong
Abstract：IBV belongs to the corona virus γ group, can cause the chicken respiratory diseases, which can cause serious losses. Now there are many methods are widely used to diagnose IBV. This study used molecular biology technique to establish Real-Time PCR method for detecting IBV, the minimum detection sensitivity was 10 copies/μL, could effectively distinguish between other avian viruses; the difference of the same plate was 0.1%~0.7% and 0.7%~1.6% in different plates, prove this method has higher sensitivity, specificity and stability. At the same time we carried infec-tion experiment about the ck/HHLJ/HH13 isolated from wild strain, and tested its concentration. The results showed that there was higher concentration in trachea, lung and kidney in chickens about ck/HHLJ/HH13, and the concentration in trachea and kidney disappeared gradually until day 21 , and their pathological changes were seriously, at last which confirmed ck/HHLJ/HH13 isolated strain was a strong virulence nephridium IBV strain.
Keywords：Infectious bronchitis virusReal-Time PCRInfectionVirus distribution
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )