Drug-resistance research and elimination of drug resistance plasmids of 10 strains of chicken source Proteus mirabilis
Su Weiyi
Li Xinnan
Han Juanzhu
Abstract：To establish a SDS method for eliminating Proteus mirabilis plasmid, understanding the relationship between plasmid and Proteus Bacillus drug resistance phenotype. To study the elim-ination conditions of the Proteus mirabilis using experimental orthogonal method, verified the methods with agarose gel electrophoresis, screened the best experiment conditions to eliminated the plasmid . Proteus drug sensitivity to 13 antibiotics was determined by microdilution method, then plasmid were eliminated. The results showed the best effective eliminate tconditions was agent concentration of SDS 0.2%, temperature 42 ℃, lasted for 72 hours. Then drug-resistance tests were took place to detect the susceptibility of Proteus with and without plasma. The results showed that, 10 drug-resistance strains of chicken source Proteus when eliminated plasmid, 9 strains were sensitive to ceftiofur.
Keywords：Proteus mirabilisPlasmid eliminationDrug resistance
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )