PCR-RFLP method for cashmere and wool fibre identification
Li Haolei
Liu Haiying
Wei Yuqing
Zhang Yiling
Li Yang
Abstract：Cashmere fibre is more expensive than wool, often adulterated during marketing. So it is very important to find a precise method to identify and differentiate those tow fibers. In this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was exploited to differentiate cashmere and wool fibres. The DNA in animal hair shafts has been isolated from scoured cashmere and wool fibres. The mitochondrial Cytochrome b sequences of both species were amplified by PCR using primers designed from known sequences. The polymorphism ob-served of the two species were detected by digestion the amplified products by ensonucleases SspⅠ. The RFLP profile clearly distinguishes the cashmere and wool fibres and this method can be ex-ploited to test the quality of cashmere products.
Keywords：CashmereWoolCytochrome bPCR-RFLPSspⅠ
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )