Influence of the alfalfa polysaccharide on vaccine immunization effect of Newcastle disease
Zhang Yue
Fang Linlin
Zhao Yanan
Yu Yang
Li Jingshuang
Abstract：In order to observe the immunoregulatory effects of alfalfa polysaccharide on Newcas-tle disease vaccine immunization, 120 1-day-age chickens were selected and randomly divided into four groups, 30 chickens per group. La Sota strain immunization and booster were performed at day-7and day-28. From day-7, three test group chickens were inoculated diffraction dose (10, 25, 50 μg/mL) of alfalfa polysaccharide muscle injection each day, and control chickens were inject-ed physiological saline water synchronously.At the day-14, 21, 28, 35, 42, 49 and 56, all chick-ens were blooded and the peripheral blood lymphocyte absorbance value (OD value) was detected by MTT method. The serum antibody titers of NDV were determined by hemagglutination inhibition (HI) method. As a results, alfalfa polysaccharide can improve the OD value of peripheral blood lympho-cytes transformation and the OD values most significantly with dose of 25 μg/mL, meanwhile, al-falfa polysaccharide can significantly increase the HI antibody titer in serum and maintain late high immune antibody level long time, antibody peak period showed longer than control groups. The experiment showed that appropriate doses of alfalfa polysaccharides obviously enhanced the immune effect of Newcastle disease vaccine immunization effect.
Keywords：Alfalfa polysaccharidesImmunizationEffect
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 30-34 )