Cloning and Expression of Glycoprotein G Gene from pseudorabies Virus Kaplan Strain
Zhao Pei
Wei Yuanyuan
Lan Desong
Wei Shu
Abstract：The glycoprotein G (gG) gene of pseudorabies virus Kaplan strain was amplified by PCR followed by sequence analysis. The results revealed that the gene fragment was 1314 base pairs (bp) in length. The full-length gG gene was cloned into the prokaryotic expression vector pET-30a(+) , and expressed in E.coli induced by IPTG. The molecular weight of the gG protein was approximately 54ku identified by SDS-PAGE and Western-Blot.The results are helpful for investigat-ing the structure and function of gG protein and preparation of diagnostic reagents for pseudora-bies.
Keywords：Pseudorabies virusGlycoprotein GGene expression
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )