Cloning and sequencing of virulence-associated factor CPS gene of Streptococcus suis type 2 isolated from pigs in Guangxi
Qin Fangyun
Bai Yun
Feng Shuping
Hu Liping
Ma Lin
Yang Rong
Xiong Yi
Abstract：Amplified and cloned by PCR, then sequenced the virulence-associated factor cps2J gene of 17 strains of type 2 Streptococcus suis isolated from Guangxi. The homologies of nucleo-tide and predicted amino acids of 17 Guangxi SS2 are 91.9%~99.8% and 89.1%~99.7% respectively.17 SS2 strains could be divided into two groups as I (11 strains isolated from healthy pigs and 5 strains from diseased pigs) and II (1 strain isolated from healthy pigs). The homologies of group I were very high (96.8%~99.8% nt and 94.6%~99.7% aa). Compared to reference strains(Netherland/ZYS8/SC17/98015), homologies of group I were 96.4%~99.9%nt and 94.1%~100%aa , 91.3%~92.4%nt and 89.1%~91.0%aa in group II. There is a low degree of variation in cps2J gene in 17 SS2 isolated from pigs in Guangxi ,China.
Keywords：Streptococcus suis type 2CPS geneCloned and sequencedHomology analysis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6-11 )