Effect of phytosterol on the serum reproductive hormone and progesterone receptor in mammary tissue of ovariectomized KM mice

Hao Haixin; Xie Xinmei; He Jianbin

doi:10.3969/j.issn.1672-9692.2014.05.002

Abstract：Experiment was conducted to study the effect of Phytosterols on the serum hormone and progesterone receptor in ovariectomized KM mice. 40 female mice was randomly selected from 50 for ovarian surgery, and was postoperative recovered for 7 days. The mice was grouped as follows: nor-mal, ovariectomized control group, phytosterols low, medium and high dose groups (daily fed with 20mg/kg, 80mg/kg, 320mg/kg phytosterols respectively). Mice were intragastric administered contin-uous for 3 weeks, blood samples and the breast tissue were collected on the 22nd day, the levels of serum estradiol, progesterone, prolactin were detected, fluorescence quantitative detection for progesterone receptor gene expression in breast tissue was conducted.The results showed that: Es-tradiol level in ovariectomized control group was significantly lower than the normal group (P<0.05). Serum estradiol level of phytosterols continue to rise, among them, serum estradiol levels of high dose group mice was the most closet to the normal group; Compared with the ovariectomized control group, serum progesterone levels in phytosterols ovariectomized mice were increased, but the difference was not significant (P>0.05); Compared with the ovariectomized control group, se-rum prolactin levels in phytosterols ovariectomized mice rised after the first decrease, but there was no significant difference between them (P>0.05);Compared with the ovariectomized control group, progesterone receptor gene relative expression level of ovariectomized mice in each dose group raised, the progesterone receptor gene relative expression levels of low, medium and high dose groups were 1.05 times, 1.13 times and 2.78 times than the ovariectomized control group. The results indicated that phytosterols can improve the imbalance of the endogenous hormones in ovari-ectomized mice, promote the expression of PR genes in breast tissue.