Effect of phytosterol on the growth and reproductive hormone of KM female mice

Hao Haixin; Xie Xinmei; He Jianbin

Abstract：The experiment was conducted to study the effects of phytosterol on growth and repro-ductive hormone in female mice. A total of 50 35-day-old female mice are adapted for one week and randomly divided into 5 groups respectively: control group (0.1 mL vegetable oil), phytosterol low, medium, high of three dose groups(daily intragastric 20, 80, 320 mg/kg phytosterol). After three weeks of Intragastric administration, body weight of mice at the first day and the twen-ty-second day were recorded, then blood from the eyeball were collected to prepare serum, serum levels of estradiol, progesterone, prolactin were detected. The influence of different levels of phytosterol on the growth and reproductive hormone in mice were analyzed. The results showed that:Compared with the control group, the body weight gain of vegetable group were significantly differ-ent(P<0.05). Compared with the vegetable group, body weight gain of phytosterols treatment group had first increased and then decreased, the difference between them was not significant(P>0.05). Phytosterol could improve the serum levels of E on different degrees2, medium dose group had the highest estradiol levels(270.52 ± 18.10 pmol/L). With the increase ofthe doses of phytosterols ga-vage, progesterone levels of mice declined in different degrees.The serum progesterone levels of phytosterol medium dose group was significant lower than the control group(P<0.05). Compared with the control group, Prolactin levels of phytosterols mice group had no significant difference(P>0.05). Low dose phytosterol can improve the body weight gain of KM female mice, Phytosterol can im-prove serum estradiol levels of KM female mice, but the level of progesterone and prolactin were not significant.