Preparation of the Specific Polyclonal Antibody Against Chinese Sacbrood Bee Virus
Fan qiong
Fei Dongliang
Ma mingxiao
Abstract：To develop a safe, effective and quality-controllable antibody against Chinese sac-brood virus for preventing and treating Chinese sacbrood virus. Suspected been larvaes were identi-fied by RT-PCR technology, and positive samples were purified by CSBV, and virus concentration were detected by BCA, then diluted to the concentration of 0.25 mg/ml. Chinese sacbrood virus are inactivated by 0.3% formaldehyde, and oil emulsion inactivated vaccine were produced after sterili-ty, safety, and stability determination. Then the New Zealand White Rabbit were injected with the vaccine for 3 times, once two weeks in order to analysis the polyclonal antibody against Chinese sacbrood virus. The results showed that the OD of the antibody titer is as high as 1.109. The high titer vaccine is prepared,and the research provide foundation for the prevention of CSBV.
Keywords：CSBVInactivated vaccinePolyclonal antibody
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 34-37 )