Simultaneous determination of many antibiotics in feedstuff by High Performance Liquid Chromatography
Li Li
Wang Jinjing
Chen Lijiang
Liu Yu
Wang Jing
Yan Xichen
Abstract：In our study, we established an efficient method for simultaneous detec-tion of 5 antibiotics in feed by high-performance liquid chromatography was estab-lished. Chlortetracycline(CTC), chloramphenicol(CAP), erythromycin(ERY), roxithromy-cin(ROX) and benzylpenicillin potassium(PG) were separated thoroughly by gradient elution under the conditions of column temperature at 30 ℃, flow rate of 1.0 mL/min, and with the injection volume of 20 μL. The signal was detected at 210 nm. The results showed that a linear range was found between peak height and concentration in the range of 0.5~40 μg/mL for CTC, CAP and PG, and 0.5~80 μg/mL for ERY and ROX. The linear regression coefficients were not less than 0.999 7. The recovery rates of the five antibiotics were from 95.86% to 98.72%, and RSD was less than 2%. High-performance liquid chromatography is simple, rapid and has high accuracy and precision, which meets the detection of CTC, CPA, ERY, ROX and PG in feedstuff.
Keywords：HPLCChlortetracyclineChloramphenicolErythromycinRoxithromy-cinBenzylpenicillin potassium
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 44-48 )