Isolation, identification and phylogenetic analysis of H1N1 swine influenza virus from pigs in Liaoning province
Lan Desong
Zhao Fengju
Wei Shu
Yan Mingmei
Gu Zhida
Gu Guibo
Hu Jun
Abstract：In our study, we isolated a swine influenza virus from pigs in an abattoir in Liaoning province. The virus was identified as H1N1subtype by HA-HI test and RT-PCR, and named A/swine/Liaoning/01/2012 (H1N1). All of the cDNAs of the eight vi-ral RNA segments were amplified by RT-PCR, sequenced, analyzed using the DNAStar soft-ware. The amino acid sequence of the HA gene near the cleavage site was IPSIQSR↓G, which was consistent with that of low pathogenic influenza virus. Genomic sequence analysis showed that all of the eight gene segments of A/swine/Liaoning/01/2012 (H1N1)had the highest homologous with the A/swine/Jiangsu/40/2011(H1N1)strain. Phy-logenetic analysis showed that the isolate was in the European avian-like SIV lin-eage. Since human infections with avian-like SIV has been reported worldwide, the iso-lation of Sw/LN/01/12 for the first time in Liaoning province has an important value for pig industry and public health, and further research of the virus is underway..
Keywords：Swine influenzaH1N1Iaolation and identificationPhylogenetic analy-sis
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 24-28 )