Optimization of ST Cell Culturing Condition for the Cultivation of Porcine Parvovirus M2 Strain
Tang Manhua
Chen Ruiai
He Ling
Li Chunhong
Liang Guiyi
Liao Cuiyin
Chen Zhenbo
Tong Ge
Abstract：To explore the suitable condition for culturing Porcine Parvovirus M2 Strain in ST Cells, synchronous inoculation and single-layer vaccination were be taken to culturing PPV-M2 strain. Then, the more effective method was choosed to optimize the culturing condition of PPV-M2 Strain in ST cells. Results showed that the method of synchronous inoculation has more advantages compared with sin-gle-layer inoculation method for culturing PPV M2 Strain. And The PPV M2 strain would propagate effectively in ST cells when controlling the density in 3.2 × 104~1.5×105/mL,using DMEM with 5% fetal bovine serum or 6% new-born calf serum as cul-ture medium,harvest the virus within 80% of the cells appearing CPE. This research could give data support for the preparation of high titer PPV antigen of M2 Strain.
Keywords：Porcine parvovirusST cellCulture condition
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 55-60 )