Analysis of estrogens residues in milk by gas chromatographic-tandem mass spectrometry
Wang Lina
Tian Xiaoling
Chen Yuyan
Abstract：This research to develop and validate a rapid sensitive and specific meth-od for quantitative analytes of estrogens residues(DES, Diethylstilbestrol: E2, Es-tradiol: EE2, Ethynylestradiol: EV, Estradiol Valerate)in milk by gas chromatograph-ic-tandem mass spectrometry and be applied to estrogens residues in milk studies. The 4 kinds of components were extracted from homogenized samples with ethyl acetate fol-lowed by solid-phase extraction with HLB column and trimethylsilylation at 70 ℃ for 40 minutes, then the GC-MS method in selected ion monitoring mode(SIM)was used to an-alyze them. A good linearity was achieved with the coefficient r2 > 0.997 8(n =2). The average recovery of estrogens was more than 74.2% with RSD lower than 9.2%(n = 9). The method is sensitive, accurate, rapid, and suitable for the analysis estrogens resi-dues in milk.
Keywords：GC-MSMilkDiethylstilbestrolEstradiolEthinylestradiol
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 38-42 )