Application by suppression subtractive hybridization in the study on viable but non-culturable state bacteria
Li Wenbo
Tan Pingping
Li Ying
Yuan Xiang
Wang Jiahui
Abstract：cDNA library constructions are basic tools of detecting new gene and re-searching gene function in gene separation and cloning in recent years. Suppression Subtractive Hybridization is one of the most efficient method in isolating differen-tially expressed genes between viable but non-culturable state bacteria and normal strains, SSH is the important way in studying virulence, pathogenicity, drug resis-tance and genetic differentiation relations of strains in VBNC bacteria. The objec-tive of this paper was to discuss the construction strategies of cDNA by SSH in pro-karyotic organism and its application to isolate differentially expressed genes in VBNC bacteria, and it put forward the outlook for further research.
Keywords：cDNA librarySuppression Subtractive HybridizationViable but non-cul-turable state
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 61-66 )