Research on granulosa cells apoptosis characteristics of atresia folliculi on Liaoning cashmere goat
Han Di
Guo Dan
Jiang Huaizhi
Ji Shuang
Yang Baozhong
Abstract： The experiment using traditional methods, TUNEL in situ method and DNA gradient electrophoresis, we study follicle's number in ovarian in estrus cycle, the apoptosis proportion of granulosa cells, and the steroid hormone levels in se-rum and follicular fluid and relation. Also the distribution and style of apoptosis granulosa cells in atretic follicles. The results revealed: the number of follicles in the ovarian varied as estrous cycles changed. DNA ladder pages show that the apop-tosis proportion of granular cells in healthy follicles, slightly astresia folli-cles and completely astresia follicles are different, they are 13.21%± 1.23%, 32.13%± 2.13%,51.31%± 3.02% respectively, as the TUNEL in situ label of granulosa cell showed. The steroid hormone levels are different in different healthy folli-cles. The E2 level is high and P4 level is low in healthy follicles. E2 level is low and P4 level is high in astresia follicles. The value of E2/P4 in the three kinds of follicles are 2.62±0.03,0.074±0.000 3,0.028±0.000 4 respectively.
Keywords：Atretic folliclesGranulosa cellsCell appotosisLiaoning cashmere goat
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 47-52 )