Determination ofβ-receptor agonists in animal urine by Ultra Performance Liquid chromatography-tandem mass spectrometry
Wang Lina
Liu Kai
Du Bailin
Liu Xiao
Liu Jingxian
Liu Ying
Abstract： A method of ultra performance liquid chromatography-tandem mass spec-trometry (UPLC-MS/MS)for simultaneous determination of nine β-receptor agonists, such as phenylethanolamineA,salbutamol,terbutaline,ractopamine,clenbuterol,bromb-uterol,mabuterol,cimbut-erol and penbutolol in animal urine was developed.The β-re-ceptor agonists was extracted with ammonium acetate,then cleaned up with a mcx sol-id-phase extraction(SPE)column.The eluate was evaporate to driness with nitrogen. The limits of detection were 0.1 μg/L for all β-receptor agonists and the limits of quantification were 0.2 μg/L,the average recoveryies were 60.63%~94.12%.The rel-ative standard deviations were 1.26%~14.74%,the real sample tests showed this meth-od can be used for the sensitive and accurate determination of multi-β-receptor ag-onists residues in animal urine.
Keywords：UPLC/MS/MSβ-receptor agonistsAnimal urine
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 42-46 )