Shihezi region field strains of canine parvovirus by PCR and preliminary study of genetic variation
Abstract：To explore the differences of the field strains of CPV in Shihezi, the study collected Shihezi area and the natural incidence of dog droppings and dead dog organiza- tion, extract the virus genome, the use of PCR incanine parvovirus VP2 gene fragment was amplified, the amplified fragments were cloned and sequenced, strains with different geo- graphical VP2 gene for comparison, analysis of their differences. In this study, VP2 gene was successfully cloned, andsequence analysis revealed a point mutation in strains exist 114 sites and five of its lack of sites, there are seven other strains of mutant sites. After genotyping, the genotype of these two strains WereCPY-2a type. The study areas of Xin- jiang Shihezi the prevalence of canine parvovirus and molecular characteristics of study provides some basis.
Keywords：ShiheziCanine parvoviruswild strainsgenetic variation
Publication Date：2012-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 64-66 )