Therapeutic effects and mechanisms of blue light combined with gel for skin photoaging in mice
WANG Zhuxian
CHEN Qiuyu
ZHOU Sijie
WANG Xuan
LIU Jun
YANG Bin
Abstract：Objective To investigate the therapeutic effects and mechanisms of blue light com-bined with gel for skin photoaging in mice.Methods Twenty-five male C57/BL6 mice were ran-domly divided into five groups:blank control,model,blue light,gel,and blue light+gel(com-bination)groups,with five mice per group.Except for the blank control group,a UVA and UVB synergistic irradiation method was used to establish a photoaging model.Mice were irradiated with 1 minimal erythema dose(MED)from week 1 to week 4,followed by 2 MEDs in weeks 5 and 6.Concurrently,starting from the third week,blue light combined with gel therapy was administered on the last day of each week.The control group received no treatment.The gel group received on-ly gel treatment for 6 minutes.The blue light group received only blue light irradiation for 6 mi-nutes.The combined group received gel treatment,followed immediately by blue light therapy.After 6 weeks,dorsal skin samples were collected for hematoxylin and eosin(HE),and Masson＇s trichrome staining.Both cutaneous and serum levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and tumor necrosis factor(TNF-α)were measured using ELISA.Results After 6 weeks:The mouse skin exhibited desquamation,dryness,roughness,lesions,leathery texture,and hyperpigmentation.Either the blue light or gel group showed no significant improve-ments in skin desquamation,dryness,roughness,or leathery texture compared with the model group.Moreover,expression levels of SOD,GSH-Px,and collagen were not significantly upregu-lated compared with the model group(P＞0.05).However,the combined group showed signifi-cant improvement in wrinkles,dryness,desquamation,and leathery texture.The expression levels of SOD and GSH-Px in the skin were significantly upregulated to levels comparable to normal(P＜0.05),accompanied by a significant increase in collagen(P＜0.001).TNF-αexpression in the skin,as well as the serum levels of SOD,GSH-Px,and TNF-α,remained no significant change(P＞0.05).Conclusions Blue light combined with gel can alleviate oxidation,reduce inflammation,and increase collagen in photoaged skin,providing experimental evidence for its clinical application in photoaging management.
Keywords：skin photoagingblue lighthydrogelsuperoxide dismutasecollagen
Publication Date：2026-01-28
Online Publishing Date：2026-03-11(First online date of this platform, not the publication date of the document)
Pages：8( 1-8 )