Characteristics of the skin microbiome in patients with atopic dermatitis:Based on 16S rDNA sequencing analysis
KE Yu
ZHANG Jianfeng
XU Yubin
CHEN Guirong
Abstract：Objective To compare the differences in skin microbial composition and diversity between patients with atopic dermatitis and healthy individuals,to provide clues for understanding the microbe-related molecular mechanisms of AD.Methods Skin samples were collected from 81 patients with atopic dermatitis(AD group)and 63 healthy individuals(control group).DNA was extracted using the CTAB method,followed by 16S rDNA sequencing.Differences in skin micro-biota were analyzed using alpha diversity,beta diversity,and principal coordinates analysis(PCoA).The composition of the skin microbiota was examined at the phylum and genus levels,and heatmaps were generated for the top 30 most abundant bacteria at both levels.Results There were differences in the microbiome between the control group and the AD group.Compared with the control group,the AD group showed increased relative abundance of Firmicutes and Staphylo-coccus,while Actinobacteria,Proteobacteria,Rhodococcus,and Lactobacillus showed decreased relative abundance.Conclusions There are significant differences in the skin microbiota between patients with AD and healthy individuals.Further research is needed to elucidate the mechanisms of microbial dysbiosis,paving the way for targeted therapeutic interventions for AD.
Keywords：atopic dermatitisskin microbiomemicrobial dysbiosis
Publication Date：2025-08-28
Online Publishing Date：2025-09-23(First online date of this platform, not the publication date of the document)
Pages：7( 571-577 )