Metabolomic profiling of cerebrospinal fluid in neurosyphilis:identification of potential diag-nostic biomarkers
HE Yuanyuan
WU Jiaxin
CHEN Zhaoqi
DENG Ziliang
WANG Haiying
KE Wujian
Abstract：Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neu-rosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chro-matography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal compo-nent analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl gly-cine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudiflo-ramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further inves-tigation into their roles in the pathogenesis of neurosyphilis.
Keywords：neurosyphilismetabolomic profilingcerebrospinal fluiddiagnostic biomarkers
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 801-809 )