Significance of Bcl-2、CD10、CD34 and CK15 expression in differentiating basal cell carcinoma from trichoepithelioma

LIU Bing-mei; XING Rong-gui; XIA Li-jie; LI Li; ZHANG Han-wen; CHENG Xue

doi:10.3969/j.issn.1674-8468.2019.01.001

Abstract：Objective:To compare expression of Bcl-2, CD10, CD34 and CK15 in basal cell carcinoma (BCC) versus trichoepithelioma (TE), and to explore the significance of their expression in diagnosis and differential diagnosis of these two disorders. Methods: Paraffin-embedded tissue samples from 36 cases of BCC and 16 cases of TE were analyzed using immunohistochemistry of Bcl-2, CD10, CD34 and CK15. The expression of these biomarkers in tumor cells and tumor stroma was evaluated. Results:The positive staining rates of Bcl-2 (palisading arrangement) in BCC and TE were 0 and 68.75％ respectively (P<0.01), while positive rates of CD10 in tumor cells of BCC and TE were 72.22％ and 12.50％ respectively (P<0.01). Moreover, positive rates of CD10 in stroma of BCC and TE were 2.78％ and 62.50％ respectively (P<0.01). Likewise, positive rates of CK15 in BCC and TE were 5.56％ and 56.25％, respectively (P<0.01).However, positive rates of CD34 in BCC and TE were comparable (P>0.05). Sensitivities were CD10 (Tumour cells+) 72.22％>Bcl-2 (68.75％)>CD10 (Stromal+) 62.50％>CK15 (56.25％);Specificities were Bcl-2 (100％)>CD10 (Stromal+) 97.22％>CK15 (94.44％)>CD10 (Tumour cells+) 87.50％;Accuracies were Bcl-2 (90.38％)>CD10 (Stromal+) 86.54％>CK15 (82.69％)>CD10 (Tumour cells+) 65.38％;Positive predictive values were Bcl-2 (100％)>CD10 (Tumour cells+) 92.86％>CD10 (Stromal+) 90.91％>CK15 (81.82％);Negative predictive values were Bcl-2 (87.80％)>CD10 (Stromal+) 85.37％>CK15 (82.92％)>CD10 (Tumour cells+) 58.33％.Conclusions: Differentiated expression and distribution of Bcl-2, CD10 and CK15, but not CD34, in BCC and TE can benefit the differential diagnosis of these two tumors.