Effect of oleanolic acid on proliferation of human hair follicle stem cells in vitro
LIU Ben
CHEN Xian-yan
YI Huan
HAN Le
DENG Wen-jia
WAN Miao-jian
Abstract：Objective:To investigate the effect of oleanolic acid (OA) on proliferation of human hair follicle stem cells (HFSC).Methods:HFSC were isolated and cultured in vitro and identified by fluorescent staining.OA was added to medium at final concentration of 1×10-4 μg/mL,1×10-3 μg/mL, 1×10-2 μg/mL, 0.1 μg/mL and 1 μg/mL, then MTT assay was performed when the cell culture was treated with OA at 24 h and 48 h to assess the number of viable cells.Results:Cultured cells were multipolar in shape and grew like typical cobble stone.The staining of K15 was positive, which is an epithelial stem cell marker.Positive rate of K15 is (86.7±5.2)% (n=4).Compared to the vehicle control group, OD490 of 1×10-4 μg/mL and 1×10-3 μg/mL of OA treated group were significantly increased (P<0.05).Conclusion:OA could stimulate proliferation of human hair follicle stem cells in vitro.
Keywords：oleanolic acidhuman haifolliclstem cellsproliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 68-71,76 )