Susceptibility of Clinical Isolates of Chlamydia Trachomatis to Azithromycin and its Resist-ant Genes in Vitro
CHEN Wen-tao
XUE Yao-hua
MAI Zhi-da
ZHENG Bi-ying
ZHENG He-ping
Abstract：Objective:To detect the sensitivity to Azithromycin and common resistance genes of Chlamydia trachomatis ( CT ) clinical isolates in Guangzhou .Methods: 111 CT clinical isolates cultured by McCoy cell lines were collected .Genes related to azithromycin resistance of the clini-cal isolates, including 23S rRNA gene, ribosomal protein L4 gene and L22 gene, were amplified through polymerase chain reaction and analyzed by agarose gel electrophoresis and sequencing . Sensitivity to azithromycin of the 46 randomly selected clinical isolates was investigated .Results:There were no resistance mutations in the 111 CT.The minimum inhibitory concentration ( MIC) of azithromycin for the 46 Chlamydia trachomatis is 0.08 ~0.32 μg/mL, while MIC50 , MIC90 were 0.08 mg/mL, 0.16μg/mL, respectively.Conclusions:The Chlamydia trachomatis clinical isolates were sensitive to azithromycin and no common resistance mutation in these CT was found in Guangzhou .
Keywords：Chlamydia trachomatisAzithromycinSusceptibility testResistance genes
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 148-151 )