Optimization and Application of PCR Detection for Mycobacterium Leprae
YE Li
LV Cheng-zhi
JIN Ya-li
WANG Hui-xiang
ZHANG Zhen-guo
SONG Shun-peng
Abstract：Objective:To establish a molecular biological method with high sensitivity for de-tecting Mycobacterium leprae(ML) in clinics.Methods:Establish a highly sensitive PCR reaction system by optimizing the reaction conditions of PCR reaction system using the pure DNA of Myco -bacterium leprae and compare the efficacy and significance of two systems-conventional PCR ( cPCR) and real-time PCR as clinical detection methods .Results:The results indicated that 10 -1 ML/mL is the lowest DNA concentration which can be steadily detected when the primer content is 0.2 μL ×100 μmol/L.The number of positive results of cPCR (49/52) was slightly higher than that of Real-time PCR ( 47/52 ) according to the results detected from the samples of leprosy pa-tients.Conclusion:Through the optimization of reaction conditions in the PCR reaction , such as DNA template concentration and primer concentration , the sensitivity of Mycobacterium leprae DNA detection can be improved .The cPCR and Real-time PCR were both highly sensitive meth-ods.However, Real-time PCR detection is more simple and rapid than cPCR , while cPCR is slightly more accurate and sensitive compare to Real-time PCR.
Keywords：Mycobacterium lepraePCRReal-time PCR
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 23-26 )