The Effect of Xianglian Solution on Candida Albicans Biofilms Model in Vitro and it′s Metabolomics Study
YUAN Juan-na
FAN Rui-qiang
XIE Ting
CHEN Xin-sheng
Abstract：Objective:To study the antifungal efficacy and the probable mechanism of Candida albicans biofilms in vitro by Xianglian Solution, in the view of metabolomics.Methods:C.albi-cans biofilms in vitro were established, and the SMIC50 and SMIC80 of the C.albicans biofilms in different maturity (4 h, 24 h and 48 h) were measured by XTT.Through the method of UPLC-Q-TOF-MS, the metabolomics of the planktonic,early (4 h),medium term(24 h),and maturity(48 h) biofilms were detected.Results:The effect of Xianglian Solution,the SMIC50 of early (4 h), medium term(24 h) and maturity(48 h) biofilms were 7.81,125 and 500 mg/mL respectively. The SMIC80 were up to 31.25,250 and >1 000 mg/mL respectively.For Fluconazole,the SMIC50 of early (4 h),medium term(24 h) and maturity(48 h) biofilms were 32,64 and >1 024μg/mL respectively,while the SMIC80 were up to 64,128 and >1 024μg/mL respectively.The metabolic profiling of the planktonic among Group Xianglian Solution,Group Fluconazole and control group were different from each other.The metabolin Tyrosyl-Arginine and Pentosidine were identified as the potential metabolin .Conclusions:Xianglian Solution was effective to C .albicans biofilms. Sugar metabolism and changes in amino acid metabolism pathways may relate to the biofilms for-mation.
Keywords：Candida albicans biofilmsMetabolomicsXianglian Solution
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 179-184 )