A Mouse Model of Immunological Contact Urticaria by Anti-dinitrophenol IgE Combined with 2, 4-Dinitrofluorobenzene
LI Run-xiang
FENG Cheng-en
LIANG BI-hua
TIAN Xin
ZHU Hui-lan
Abstract：Objective:To establish an animal model of immunological contact urticaria of mice . Methods:Thirty-six BALB/c mice were randomly divided into 3 groups.The mice of model group were injected with anti-dinitrophenol IgE monoclonal antibody by tail vein .The mice of control groups were injected with anti-dinitrophenol IgE monoclonal antibody or normal saline by tail vein . After 24 hours, 2, 4 -dinitrofluorobenzene ( DNFB) was painted on the ears of model group and physiological saline was painted on the ears of control groups respectively .Mice were observed within 14 days of the wheal, pruritus, ear thickness, serum levels of IgE, IL-4 and IFN-γ, hista-mine and skin pathology .Results:Wheals were seen on the mice of all of mode group .Compared with control groups , scratching number , ear thickness and serum levels of histamine of the mice of model group were increased significantly (P<0.01).However, there were no differences in ser-um levels of IgE , IL-4 and IFN-γamong 3 groups ( P>0.05 ) .Conclusion:A mouse model of immunological contact urticaria would be quickly and reliably established by induction of anti -dini-trophenol IgE monoclonal antibody and DNFB .
Keywords：Immunological contact urticaria modMouseAnti-dinitrophenol IgE mono-clonal antibodyDinitrofluorobenzene
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 25-28,32 )