Establishment of the Cell Model for Persistent Infection of Chlamydia Trachomatis Intro-duced by IFN-γin vitro
XUE Yao-hua
BAI Shun
ZHENG Lei
QIN Xiao-lin
ZENG Wei-ying
HUANG Jin-mei
WU Xing-zhong
OU Jiang-li
ZHENG He-ping
Abstract：Objective:To establish a cell model of interforn-γmediated persistent Chlamydia trachomatis infection in vitro. Methods:McCoy cells were prepared in culture plates and Chlamydia trachomatis serovar D were incubated for infection .Infected cells with treated or un-treated IFN-γat 0.5 ng/mL for 72 hours and were harvested .Light microscope , fluorescence mi-croscope and electron microscope were used for structural analysis .The culture medium was then removed and replaced with RPMI 1640 for 48 hours after the removal of IFN-γ.The morphological characteristics of the Chlamydia were observed .Results:For iodine stained assay , the infected cells mediated by IFN-γcontained small numbers of inclusions by light microscope .Inclusions lacked of density and the fluorescent dye in the IFN-γmediated inclusions were uneven through di-rect immunofluorescent staining .Typical RB and EB formed in inclusions of untreated cells were observed by electronic microscope .The IFN-γtreated cells did not contain typical forms , but in-stead the inclusions were characterized by large atypical reticulate body .Conclusion:An in vitro cell culture system for IFN-γmediated persistent Chlamydia trachomatis infection have been estab-lished .The persistent infection cell model was the foundation for the mechanism research of persis-tent Chlamydia.
Keywords：Interforn-γChlamydia trachomatisPersistent infectionCell model
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 10-13,17 )