The Expression and Tissue Distribution of Protease Activated Receptor-2 in Prurigo Nodu-laris
LI Yang-qi
LU Chun
TIAN Xin
MA Shao-yin
Abstract：Objective:To investigate the expression and tissue distribution of protease activated receptor-2(PAR-2) in patients with lesions of prurigo nodularis (PN),and to study the role of PAR-2 in the development of pruritus in PN .Methods:Skin tissue samples were collected from 20 healthy controls and 22 patients with PN .Immunohistochemical SP staining was carried out to de-tect the expression level and distribution of PAR-2 in skin tissues of patients with lesions group , non-lesional group and normal control group .The expression level in three groups were compared with each other .Visual analogue scale was used to calculate the subjective degree of itching of 22 patients with PN , and the correlation between visual analogue scale and PAR-2 expression level were analyzed .Results:Normal skin tissues , lesions and non-lesional skin tissues of prurigo nodu-laris were positively stained by PAR-2.The cytoplasm and membrane of keratinocytes in epidermal acanthosis and basal layer were the mainly expressing sites .The expression level of PAR-2 was significantly higher in patients with lesions group than in the healthy controls and the non -lesional group of PN (t=2.34, 2.10, P<0.05).Subjective degree of itching of 22 patients with PN u-sing visual analogue scale was correlated positively with the expression level of PAR -2 in lesion of patients of PN ( r =0.62, P <0.05 ) .Conclusion:PAR-2 was closely related with the pruritus pathogenesis in PN .
Keywords：Prurigo nodularisImmunohistochemical stainingProtease activated receptor-2Pruritus
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 367-371 )