Proteomics Study of T Lymphocyte Membrane in Psoriasis Vulgar with Different TCM Syndrome Types by MALDI-TOF-MS Technology
WU Wei
FAN Rui-qiang
YAN Hui
WU Hui-mei
YAO Dan-ni
Abstract：Objective:To search proteins that might serve as T lymphocyte membrane biomark-ers for different TCM syndrome types of psoriasis vulgar by analyzing the proteomic components of T lymphocyte membrane from patients with psoriasis vulgar and normal controls .Methods:The anticoagulated blood from 30 patients with psoriasis vulgar including 10 blood-stasis syndrome、10 blood-heat syndrome and 10 blood-dryness syndrome and the anticoagulated blood of 10 normal people were collected to sort out T lymphocytes , and T lymphocyte membranes were extracted . The proteomics were detected by matrix-assisted laser desorption/ionization time of flight mass spectrometry ( MALDI-TOF-MS ) .Results:Four differentially expressed protein spots were ob-served between patients with psoriasis vulgar and normal controls .One differentially expressed pro-tein spot between blood-heat syndrome and blood-stasis syndrome and another one between blood-stasis syndrome and blood-dryness syndrome were successfully identified .No differentially ex-pressed protein spot was found between blood-heat syndrome and blood-dryness syndrome .Conclu-sion:There are some differences between T lymphocyte membrane proteins obtained from the pa-tients with psoriasis vulgar of different TCM syndrome types and normal controls , but more samples will be needed to confirm the results in future studies .
Keywords：Psoriasis vulgarMALDI-TOF-MSTCM syndrome typeLymphocyte membrane protein
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 363-366 )