Study on Differential Expression Profile of Transcribed Ultra Conserved Region in PBMC of Patients with Psoriasis Vulgaris
GAO Qian
ZHOU Hui
LI Wen-jing
Abstract：Objective:To investigate differential expression profile of transcribed ultra con-served region ( T-UCR) in PBMC of patients with psoriasis vulgaris by microarray analysis .Meth-ods:Blood samples for extraction of total RNA in PBMC were collected from 7 patients with psoria-sis vulgaris and 3 healthy individuals.mRNAs and LncRNAs were purified from total RNA.Then, each sample was amplified and transcribed into fluorescent cRNA .The labeled cRNAs were hy-bridized onto the human T-UCR Array.After the slides were washed , the arrays were scanned by the Agilent Scanner G2505C.Results:A total of 109 T-UCRs were found expressed in PBMC . Compared with T-UCR in healthy individuals , 1 was upregulated and 4 T-UCRs were down-regula-ted in patients with psoriasis vulgaris .A total of 2 043 novel T-UCRs were found expressed in PB-MC.Among them, 100 was upregulated and 82 were down-regulated in patients with psoriasis vul-garis.Both of 232 RNA transcripts whose transcription Units overlap UCRs and 67 UCR-proximal genes were significantly differentially expressed .GO analysis revealed that 36 differentially ex-pressed RNA transcripts whose transcription Units overlap UCRs were enriched in cellular process , and 5 of them were related to T cells .Conclusion:T-UCR may play an important role in the path-ogenesis of psoriasis vulgaris .
Keywords：Psoriasis vulgarisTranscribed ultra conserved tegionMicroarrayDiffer-ential expression
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 357-362 )