The Effects of Dexamethasone on Expression of Pattern Recognition Receptors and Inflam-matory Cytokines in Macrophage Infected by F.monophora
CAI Wen-ying
LU Chang-ming
LI Xi-qing
JIANG Li
SUN Jiu-feng
Abstract：To explore the effects of dexamethasone to the expression of pattern rec-ognition receptors ( PRRs ) and inflammatory cytokine production by macrophage in an in vitro model of infection.Methods:Four groups were established to evaluate the effects of DEX to PRRs, including F.monophora+DEX+macrophage group, F.monophora+macrophage group, DEX+macrophage group and negative control groups.The mRNA expression levels of Dectin-1, TLR-2, TLR-4 were determined by real time PCR.The secretion of TNF-αin culture supernatant was determined by ELISA.The groups including DEX only and normal macrophage were used as control.Results:The expression of Dectin-1 increased significantly in the F.monophora+macro-phage group, but decreased after DEX was added in the F.monophora+DEX group( t=121 900, P<0.05) .The expression of TLR-2 increased in the F.monophora+DEX group, but not signifi-cant statistically(t=3 022, P>0.05).The expression of TLR-4 was down regulated in F.mono-phora+macrophage group, while no difference was detected when DEX was added ( t=2 021, P>0.05).The secretion of TNF-αwas decreased significantly in F.monophora+DEX group in comparison to F.monophora infection group (t=1 514 000, P<0.05).Conclusion:DEX most likely down-regulated the expression of Dectin-1 and the secretion of TNF-α, which resulted in im-mune tolerance in F.monophora infected macrophage in this in vitro model of infection.Yet, no significant effects were observed in expression levels of TLR-2、TLR-4 statistically.
Keywords：DexamethasoneF.monohporaPattern recognition receptorsDectin-1TLR-2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 173-176,180 )