Investigation on HaCaT Cells Inducing Marrow Mesenchymal Stem Cells from Rabbit to Express Cytokeratins
LI Wei
LI Song-ze
Abstract：Objective:To investigate the possibility of inducing marrow mesenchymal stem cells ( MSCs) from rabbit to express cytokeratin by co-culturing with HaCaT cells in vitro.Methods:MSCs were isolated and identified by detecting the expressions of CD 34,CD44,CD45 with flow cy-tometry,then the MSCs were co-cultured with HaCaT in vitro.After co-cultured for 3 and 7 days, the MSCs and HaCaT cell were separated , and Pan-cytokeratin and CK19 were detected by immu-nocytochemistry respectively .Results:Flow cytometry showed negative reaction in primary culture cells for CD34,CD45 and positive for CD44, which proved these were MSCs .After Co-cultured for 3 and 7 days, immunohistochemistry revealed that MSCs were all positive for Pan-cytokeratin (Pan-CK) and CK19.The positive rate of Pan-CK of the MSCs which co-cultured with HaCaT cell for 7 day as higher than that for 3 days,which was statistical significantly (29.1 ±8.3%, 13.5 ±3.7%, t=13.35, P<0.05) .While the difference of the positive rate of CK 19 between the two groups of MSCs co-cultured with HaCatT cell for 7 and 3 days showed no statistical signifi-cance(3.3 ±0.8%,3.1 ±0.5%,t=1.37, P>0.05).Conclusion:MSCs can express Pan-CK and CK19 by co-culturing with HaCaT cell , which indicated that MSCs might has the potential ac-tion to differentiate into epidermal stem cells .
Keywords：Marrow mesenchymal stem cellsCytokeratinHaCaT cell
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 104-107 )