Effect of 308 nm Excimer Laser on TNF-α,bFGF in the Skin Tissue Fluid of Patients with Vitiligo Vulgaris in Stable Stage

PI Xiao-bing; XIE Zhi-hong; PAN Jing-liang

doi:10.3969/j.issn.1674-8468.2014.02.002

Abstract：Objective:To evaluate the influence of the 308 nm excimer laser on the level of the tumor necrosis factor-α( TNF-α) and the basic fibroblastic growth factor ( bFGF) in the skin tissue fluid of patients with vitiligo vulgaris in stable stage .Methods:28 patients with vitiligo vulgaris in stable stage receiving autologous epidermal grafting were included in this study .The vitiliginous lesion , the perilesional skin and the normal skin were divided into laser treatment section and non-laser treatment section . Before grafting , the laser treatment section were irradiated with the 308 nm excimer laser for ten times .The suction blister fluid in different section were collected , and the level of TNF-αand bFGF of those fluids in different section were measured by the enzyme linked immunosorbent assay .Results:In vitiliginous lesion , perilesional skin and normal skin , the TNF-αlevel in the laser treatment section were lower than that in the non-laser treatment section , while the bFGF level in the laser treatment section were higher than that in the non-laser treatment section(P<0.05).The TNF-αlevel in the non-laser section in vitiliginous lesion and in perile-sional skin were significantly higher than that in normal skin ( P<0.05 ) , and the bFGF level in non-laser section in vitiliginous lesion and in perilesional skin were significantly lower than that in normal skin(P<0.05).There were no significant difference in the level of TNF-αand bFGF in non-laser section and the laser section between the vitiliginous lesion and the perilesional skin (P>0.05).Conclusion:The 308 nm excimer laser can inhibit the keratinocyte to secret TNF-αand upgrade the level of bFGF in tissue fluids , which would be one of mechanisms of the 308 nm excimer laser in treatment of vitiligo on inducing repigmentation of skin lesions .