Comparison of the Efficacy of Two PCR Technologies in Detecting Treponema Pallidum DNA from Patients with Latent Syphilis
ZHANG Sen-miao
CHEN Fang-mei
CAI Chuan-chuan
HUANG Xiao-xiong
Abstract：Objective:To compare the efficacy of two PCR methods in detecting Treponema pal-lidum ( TP) DNA from patients with latent syphilis .Methods:Samples including whole peripheral blood, plasma and serum from 40 patients with latent syphilis were collected .The 47-PCR and pol A-PCR techniques were used to detect 47-kD protein and DNA polymerase I gene fragments re-spectively .Results:Positive ratios of TP-DNA by 47-PCR for whole blood , plasma and sera were 30.0%, 37.5%and 20.0%respectively , and those were 22.5%, 27.5% and 15.0% respec-tively by pol A-PCR.No significant differences were observed between different methods for the a-bove mentioned samples .Conclusion:Both the 47-PCR and pol A-PCR can be used as adjuvant diagnostic tool to screen TP-DNA in patients with latent syphilis .
Keywords：Latent syphilisTreponema pallidumPCR
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3-6 )