Antimicrobial Resistance in 525 Clinical Isolates of Neisseria gonorrhoeae from 5 Cities in Guangdong
WU Xing-zhong
HUANG Jin-mei
LIU Xiao-feng
HUANG Shu-jie
CHEN Shao-nan
GUO Zhi-xing
LI Yan-wa
QIN xiao-lin
XUE Yao-hua
ZENG Wei-ying
TANG San-mei
LAN Yin-yuan
LI Yi-xin
ZHENG He-ping
Abstract：Objective:To study the current status of antimicrobial resistance to 6 antimicrobial agents of clinical isolates of Neisseria gonorrhoeae from 5 cities in Guangdong province in 2011~2012 .Methods:The agar dilution method was used to determine the minimum inhibitory concen-trations ( MICs) to six antibiotics, i.e., penicillin G, tetracycline, ciprofloxacin, cefixime, ceftriaxone and spectinomycin .The resistance of all strains to six antibiotics was interpreted ac-cording to criteria used in the project of surveillance of gonococcal antibiotic susceptibility in the WHO Western Pacific Region and cefixime susceptibilities were interpreted as Clinical and Labora-tory Standards Institute ( CLSI ) . Penicillinase (β-lactamase ) -producing N. gonorrhoeae ( PPNG) was analyzed by the paper acidometric method .Results:525 strains N.gonorrhoeae iso-lates collected from Guangzhou , Foshan, Jiangmen, Zhaoqing and Zhuhai in Guangdong province were studied .430 isolates ( 81.9%) showed resistance to Penicillin .The prevalence of tetracy-cline-and ciprofloxacin-resistance was 92.2%and 97.9%, while none of strains appeared to be resistant to spectinomycin , cefixime and ceftriaxone , but 178 ( 33.9%) and 7 ( 1.3%) isolates showed reduced sensitivity to ceftriaxone and cefixime , respectively.MIC50 and MIC90 of penicil-lin, tetracycline and ciprofloxacin were significantly higher than resistant standards .189(36.0%) strains were PPNG and 248 ( 47.2%) strains were TRNG .Chi-square analysis showed a marked increase in the percentage of N.gonorrhoeae isolates with resistance to Penicillin in Zhaoqing city , and resistance to tetracycline in Guangzhou city .N.gonorrhoeae with reduced susceptibility to ceftraxone was isolated in Guangzhou city , prevalence of PPNG in Zhaoqing city increased com-pared with the average level ( P<0.01 ) .There was no significant difference about TRNG preva-lence rate in five cities (χ2=4.16 , P>0.05 ) .Conclusion:There is a significant difference in antimicrobial susceptibility of N.gonorrhoeae from different areas of Guangdong province .A sig-nificant elevation is observed in the percentage of N.gonorrhoeae with reduced susceptibility to ceftriaxone in Guangzhou and the prevalence of PPNG in Zhaoqing city , to which close attention should be paid .
Keywords：Neisseria gonorrhoeaeMicrobial sensitivity testDrug monitoring
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 396-400 )