Detection of the HLA-B*5801 Allele in Han Chinese with Allopurinol-induced Severe Drug Eruption

DENG Zhi-yuan; YANG Jian; YANG Wen-lin

doi:10.3969/j.issn.1674-8468.2013.06.003

Abstract：Objective:To detect the HLA-B*5801 allele of Han Chinese patients with allopuri-nol-induced severe drug eruption and evaluate the significance of HLA-B*5801 allele as a genetic marker to predict allopurinol-induced severe drug eruption in Han Chinese .Methods:HLA-B*5801 allele genotyping was performed by the polymerase chain reaction-sequence specific primers method in six patients with Stevens-Johnson syndrome ( SJS) or toxic epidermal necrolysis ( TEN) (group A) and four patients with maculopapular eruption (MPE) (group B).In addition, twenty allopurinol-tolerant patients who had used allopurinol for six months without evidence of any drug eruption (group C) and 185 healthy individuals who never used the drug and without severe drug eruption (group D) were recruited as controls.Results:The HLA-B*5801 allele were identified in all six patients (100%) of group A, one patient (25.0%) of group B, two patients (10.0%) of group C and seventeen individuals (9.19%) of group D.Compared with healthy individuals , the frequency of the HLA-B*5801 allele in allopurinol-induced SJS/TEN patients was significantly higher (χ2=45.25, P<0.05).Whileas no statistical significance was found in difference of the frequency of HLA-B*5801 allele both between allopurinol-induced MPE patients and healthy indi-viduals and between allopurinol-tolerant patients and healthy individuals (χ2=1.15,0.01 respec-tively;both P>0.05).Conclusion:In Han Chinese, there is a strong association of HLA-B*5801 allele in patients with allopurinol-induced SJS/TEN, but not in patients with allopurinol-in-duced maculopapular eruption .