Diagnostic efficacy of combined serum VASN,CD10,and NOX2 detection in differentiating colon cancer from colon polyps
ZHANG Yingying
ZHANG Xinxin
WANG Tian
QI Jingwen
LIU Yaqing
Abstract：Objective To explore the value of combined detection of serum Vasorin(VASN),cluster of differentiation 10(CD10),and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2)levels in the differential diagnosis of colon polyps and colon cancer.Methods Colon cancer patients admitted to The First People's Hospital of Shangqiu from February 2022 to February 2024 were selected as the colon cancer group(n=125),and patients with colon polyps during the same period were selected as the polyp group(n=117).Additionally,125 healthy individuals from physical examinations during the same period were selected as the control group.Baseline data were collected from all participants,and serum levels of VASN,CD10,and NOX2 were measured using enzyme-linked immunosorbent assay(ELISA).The serum levels of VASN,CD10,and NOX2 were compared.A multivariate Logistic regression model was constructed to explore the influencing factors of colon cancer.The diagnostic value of individual and combined indicators(VASN,CD10,NOX2)for colon cancer was evaluated by plotting receiver operating characteristic(ROC)curves and calculating the area under the curve(AUC).Results The serum levels of VASN,CD10,and NOX2 in the colon cancer group were significantly higher than those in the polyp group and the control group,with values of(198.76±25.96)pg/mL,(1.07±0.31)ng/mL,(15.26±2.69)ng/mL versus(169.83±18.36)pg/mL,(0.73±0.22)ng/mL,(12.49±2.25)ng/mL in the polyp group and(95.48±11.87)pg/mL,(0.32±0.08)ng/mL,(6.82±1.73)ng/mL in the control group(all P＜0.001).Among colon cancer patients,the levels of these markers significantly increased with higher TNM stages,greater depth of tumor invasion,and the presence of lymph node metastasis(all P＜0.01).Multivariate Logistic regression analysis further confirmed that elevated serum levels of VASN,CD10,and NOX2 were independent risk factors for colon cancer(all P＜0.05),with corresponding odds ratios(95％confidence intervals)of 1.998(1.059-3.770),2.034(1.051-3.937),and 2.125(1.160-3.894),respectively.ROC curve analysis demonstrated that the combined detection of VASN,CD10,and NOX2 provided excellent diagnostic performance for colon cancer(AUC=0.941,sensitivity 84.80％,specificity 88.03％),which was significantly superior to the AUCs of VASN,CD10,and NOX2 alone(0.777,0.822,0.800;DeLong test,all P＜0.001).Conclusion Serum VASN,CD10,and NOX2 are potential biomarkers for colon cancer.Their combined detection holds significant clinical application prospects in the differential diagnosis of colon polyps and colon cancer,and can provide a reference for early disease identification.
Keywords：colon cancerVasorincluster of differentiation 10nicotinamide adenine dinucleotide phosphate oxidase 2differential diagnosis
Publication Date：2026-01-28
Online Publishing Date：2026-03-18(First online date of this platform, not the publication date of the document)
Pages：6( 47-52 )