Bioinformatics analysis of the expression and clinical significance of Rho GTPase activating protein 9 in kidney clear cell carcinoma
WANG Jialong
YIN Guicao
LI Yifan
Abstract：Objective To investigate the expression,clinical prognostic value,and correlation with immune cell infiltration of Rho GTPase activating protein 9(ARHGAP9)in kidney clear cell carcinoma(KIRC).Methods Based on the The Cancer Genome Atla database,and validation with clinical samples,we compared the expression differences of ARHGAP9 between KIRC tumor tissues and normal tissues.Through stratified analysis,the relationship between ARHGAP9 expression levels and the methylation status of its DNA promoter with patients' clinicopathological characteristics was investigated.The Search Tool for the Retrieval of Interacting Genes/Proteins was utilized to construct a protein interaction network for ARHGAP9,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed on the interacting genes.Furthermore,the Tumor Immune Estimation Resource database algorithm was applied to evaluate the association between ARHGAP9 expression and immune cell infiltration levels in the tumor microenvironment.Combined with Cox proportional-hazards regression models,the independent prognostic value of ARHGAP9 expression and key immune cell infiltration in KIRC patients was assessed.Results ARHGAP9 was differentially expressed in multiple cancers and correlated with patient prognosis(P＜0.05).The mRNA and protein expression levels of ARHGAP9 were upregulated in KIRC(P＜0.05).In KIRC,ARHGAP9 mRNA and protein expression,as well as DNA promoter methylation levels,were associated with histological grade,pathological stage,and lymph node stage(P＜0.05).Functional enrichment analysis results indicated that ARHGAP9 was involved in regulating the sphingolipid signaling pathway,cAMP signaling pathway,Ras signaling pathway,and Wnt signaling pathway.The expression of ARHGAP9 correlated with the infiltration levels of CD4+T cells,CD8+T cells,neutrophils,dendritic cells,B cells,and macrophages(P＜0.05).Univariate and multivariate Cox regression analyses revealed that high expression of ARHGAP9 was an independent prognostic risk factor for KIRC patients.Conclusion ARHGAP9 is highly expressed in KIRC and associated with poor prognosis,suggesting that it may serve as a reliable prognostic biomarker and a potential therapeutic target for KIRC patients.
Keywords：kidney clear cell carcinomaRho GTPase activating protein 9clinical significanceprognosisimmune infiltration
Publication Date：2026-01-28
Online Publishing Date：2026-03-18(First online date of this platform, not the publication date of the document)
Pages：7( 25-31 )