The value of CD8⁺T cells from tumor biopsy in early prediction of the efficacy and prognosis of neoadjuvant chemotherapy for osteosarcoma
GUO Haoyu
PENG Xiuqing
LUO Yuejun
FENG Wanjiang
XU Zibo
WANG Bo
LIU Weifeng
Abstract：Objective To explore the value of CD8⁺T cell infiltration in osteosarcoma biopsy tissues before treatment in predicting the response to neoadjuvant chemotherapy(NACT)and the prognosis of patients.Methods A total of 185 patients with limb osteosarcoma admitted to Beijing Jishuitan Hospital,Capital Medical University from January 1,2021 to April 23,2025 were selected and divided into a training cohort(n=129)and a validation cohort(n=56).Biopsy tumor tissue samples of the patients before treatment were collected.The infiltration density of CD8⁺ T cells in tumor tissues was detected by immunohistochemical staining,and the patients were divided into the high infiltration group and the low infiltration group based on the median value of the infiltration density.The relationship between CD8⁺ T cell infiltration and the therapeutic effect of NACT was analyzed by the Logistic regression model.The overall survival time(OS),metastasis-free survival time(DMFS),and tumor-free survival time(DFS)of the patients were followed up.The survival curve was plotted using the Kaplan-Meier method,and the survival difference was tested by Log-rank.Results The density of CD8⁺ T cells in osteosarcoma biopsy tissues of the training cohort ranged from 0.74 to 1 322.10 cells/mm²,with a median value of 25.28 cells/mm².The verification cohort ranged from 0.70 to 548.71 pieces/mm²,with a median of 18.79 pieces/mm².Both the training cohort and the validation cohort showed that high infiltration of CD8⁺ T cells was significantly correlated with NACT sensitivity(P<0.001).The results of multivariate Logistic regression analysis showed that CD8⁺ T cell infiltration was a significant factor in predicting the efficacy of NACT(OR=0.03,P<0.001).The DMFS and DFS of patients in the high infiltration group were significantly better than those in the low infiltration group.Conclusion The infiltration level of CD8⁺ T cells in osteosarcoma tissues before treatment is closely related to the therapeutic effect of NACT.Patients with high infiltration benefit more from NACT.The infiltration level of CD8⁺ T cells can serve as a potential biomarker for predicting the efficacy of NACT.
Keywords：osteosarcomaCD8⁺ T cellsneoadjuvant chemotherapyresponsepredictionprognostic
Publication Date：2025-12-28
Online Publishing Date：2026-01-12(First online date of this platform, not the publication date of the document)
Pages：8( 1157-1164 )