Expression of integrin β1 in oral squamous cell carcinoma and its effect on proliferation,invasion and migration
WANG Zhiqiang
WANG Xichao
ZHOU Min
QIN Jia
Abstract：Objective To analyze the expression of integrin β1(ITGB1)in oral squamous cell carcinoma(OSCC)and its effects on the proliferation,invasion and migration of OSCC cells.Methods GEPIA2 and TIMER2 databases were utilized to analyze the expression of ITGB1 in head and neck squamous cell carcinoma(HNSC)tissues.A total of 42 patients with OSCC who underwent surgical treatment in the Third Affiliated Hospital of Xinxiang Medical University were included.According to the median expression level of ITGB1 protein,the patients were divided into the high ITGB1 group and the low ITGB1 group,and the relationship between the expression level of ITGB1 and the clinicopathological characteristics was analyzed.The mRNA and protein expression levels of ITGB1 in OSCC tissues and cells were detected by quantitative reverse transcription PCR(RT-qPCR)and Western blotting.The expression of ITGB1 was down-regulated by siRNA technology,and then the proliferation,apoptosis,migration and invasion abilities of OSCC cells were detected through CCK-8 assay,flow cytometry,scratch assay,and Transwell assay.Further,the expression of apoptosis and epithelial-mesenchymal transition(EMT)-related proteins was examined by Western blotting.Results The database results showed that ITGB1 was highly expressed in HNSC tissues compared with adjacent normal tissue(P<0.05).The RT-qPCR and Western blotting results showed that the expression levels of ITGB1 in OSCC tissues were higher than in adjacent cells(P<0.001).There were significant differences in the degree of tumor differentiation and TNM stage between the high ITGB1 group and the low ITGB1 group(all P<0.05).At the cellular level,the expression levels of ITGB1 in OSCC cells lines followed the order:HOK<CAL-27<SCC-25 in sequence(all P<0.001).After transfection with si-ITGB1,the expression levels of ITGB1 in SCC-25 cells were inhibited(P<0.001).Compared with the si-NC group,the proliferation activity of cells in the si-ITGB1 group at 24 h,48 h,72 h and 96 h was significantly decreased(P<0.05),a significantly increased apoptosis rate(P<0.001),accompanied by increased Bax expression and decreased Bcl-2 expression(P<0.05).The wound healing assay showed a significantly increased relative wound width at 24 h(P<0.05).The Transwell assay showed a significant decrease in the number of invading cellst(P<0.01).After co-transfection with si-ITGB1,the expression level of E-cadherin significantly increased(P<0.001),while the expression levels of N-cadherin and Vimentin significantly decreased(P<0.001).Conclusion ITGB1 is highly expressed in OSCC,and down-regulating ITGB1 expression can inhibit the proliferation of SCC-25 cells while promoting cell apoptosis.It can also inhibit the migration and invasion of OSCC cells by regulating the expression levels of EMT-related proteins.
Keywords：oral squamous cell carcinomaintegrin β1profiferationmigrationinvasion
Publication Date：2025-11-28
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：7( 1067-1073 )