Effects of plumbagin on the growth,invasion and apoptosis of gastric cancer cells by regulating the expression of HIF-1α
LI Xin
WANG Shouxing
LIU Yuangang
WEN Jinping
YANG Chaoxin
Abstract：Objective To investigate the effects of plumbagin(PLB)on the growth,invasion,and apoptosis of gastric cancer cells by regulating the expression of hypoxia inducible factor-1α(HIF-1α)and programmed death ligand-1(PD-L1).Methods Gastric cancer cells MGC-803 were assigned into control group(conventional culture),PLB group(8 μmol/L PLB),PLB+pcDNA-NC group and PLB+pcDNA-HIF-1α group,respectively.Cell proliferation,invasion and apoptosis were detected by MTT assay,plate clone formation experiments,Transwell experiment and Flow cytometry,respectively.The mRNA and protein expressions of HIF-1α and PD-L1 were detected by quantitative real time PCR(RT-qPCR)and Western blotting,respectively.Transplant tumor models were constructed based on the MGC-803 cell lines.The twenty-four mice were randomized into four groups(n=6):a control group given intraperitoneal saline,and PLB,PLB+pcDNA-NC,and PLB+pcDNA-HIF-1α groups all treated with daily intraperitoneal injections of 6 mg/kg PLB for 6 weeks.Tumor volume and weight were measured,and the mRNA and protein expression levels of HIF-1α and PD-L1 in tumor tissues were detected by qRT-PCR and Western blotting experiments.Results Compared with the control group,the cell survival rate,colony formation rate,number of invasive cells,HIF-1α and PD-L1 mRNA and protein expression levels of gastric cancer cells in the PLB group were lower,and the apoptosis rate was higher,the volume and weight of transplanted tumors and the expression of HIF-1α and PD-L1 mRNA and protein in tumor tissues were also decreased(P<0.05).Compared with the PLB+pcDNA-NC group,the cell survival rate,colony formation rate,number of invasive cells,HIF-1α and PD-L1 mRNA and protein expression levels of gastric cancer cells in the PLB+pcDNA-HIF-1α group were higher,while the cell apoptosis rate was lower,the volume and weight of transplanted tumors and the expression of HIF-1α and PD-L1 mRNA and protein in tumor tissues were also increased(P<0.05).Conclusion PLB may downregulate PD-L1 by inhibiting HIF-1α,thereby inhibiting gastric cancer cell growth and invasion,and promoting cell apoptosis.
Keywords：gastric cancer cellsplumbaginhypoxia inducible factor-1αprogrammed death ligand-1
Publication Date：2025-11-28
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：6( 1061-1066 )