The impacts of LncRNA OIP5-AS1 on the proliferation,migration and invasion of papillary thyroid carcinoma cells by modulating the miR-410-3p/MYH9 axis
ZHANG Menglei
YANG Longfei
WANG Na
Abstract：Objective To discuss the impacts of long non-coding RNA OIP5-AS1(LncRNA OIP5-AS1)on the proliferation,migration and invasion of papillary thyroid carcinoma(PTC)cells by modulating the microRNA-410-3p(miR-410-3p)/non-muscle myosin heavy chain 9(MYH9)axis.Methods Real-time fluorescence quantitative PCR reaction(RT-qPCR)and Western blotting were used to detect the expression of LncRNA OIP5-AS1,miR-410-3p,and MYH9 in PTC cell lines(IHH4,TPC-1,B-CPAP)and normal human thyroid follicular epithelial cells Nthy-ori3-1,in order to screen for the best intervention cells.TPC-1 cells were separated into Control group,sh-NC group,sh-OIP5-AS1 group,sh-OIP5-AS1+anti-miR-NC group,sh-OIP5-AS1+anti-miR-410-3p group,mimics NC group,miR-410-3p mimics group,miR-410-3p mimics+pcDNA group,and miR-410-3p mimics+MYH9 group.The method of RT-qPCR was used to detect the expression of LncRNA OIP5-AS1 and miR-410-3p of cells.Plate cloning experiments and flow cytometry were used to detect cell proliferation and apoptosis,respectively.Transwell chamber experiment was used to measure cell migration and invasion.Western blotting was used to measure the protein expression of MYH9,Ki-67,MMP-2 and MMP-9.The dual luciferase reporter genes were used to measure the targeting relationship between LncRNA OIP5-AS1,MYH9,and miR-410-3p.The nude mouse transplant tumor experiment was used to verify the effect of LncRNA OIP5-AS1 on the growth of PTC transplant tumors.Results Compared with the Nthy-ori3-1 cell,the LncRNA OIP5-AS1 and MYH9 protein expression increased in IHH4,TPC-1,and B-CPAP cells,while miR-410-3p expression decreased(P<0.05).TPC-1 cells were selected for the experiment.Silencing LncRNA OIP5-AS1 or overexpression of miR-410-3p was able to prominently upregulate miR-410-3p expression and downregulate MYH9 expression,reduce the number of clone formation,cell migration and invasion,and increase the expression of Ki-67,MMP-2,and MMP-9 proteins,and also increase the apoptosis rate of cells(P<0.05).Inhibition of miR-410-3p or overexpression of MYH9 was able to weaken the effect of silencing LncRNA OIP5-AS1 or overexpression of miR-410-3p on the malignant behaviors of TPC-1 cells(P<0.05).LncRNAs OIP5-AS1,MYH9 had a targeted regulatory relationship with miR-410-3p.Silencing LncRNA OIP5-AS1 could significantly reduce the volume,mass and expression levels of LncRNA OIP5-AS1 and MYH9 in the PTC transplanted tumors,and significantly increase the expression of miR-410-3p(P<0.05).Conclusion Silencing LncRNA OIP5-AS1 can inhibit the malignant progression of PTC by modulating miR-410-3p/MYH9 axis.
Keywords：papillary thyroid carcinomalong non-coding RNA OIP5-AS1microRNA-410-3pnon-muscle myosin heavy chain 9proliferationmigrationinvasion
Publication Date：2025-11-28
Online Publishing Date：2026-01-05(First online date of this platform, not the publication date of the document)
Pages：7( 1041-1047 )