Expression and clinical significance of P62 and PTEN in malignant melanoma tissues of nasal cavity
LI Xiaohui
XU Lihua
LI Zhiqiang
LI Xiaoli
ZHANG Weidong
WANG Jing
Abstract：Objective To investigate the expression levels of sequestosome 1(P62)and phosphatase and tensin homolog deleted on chromosome 10(PTEN)in nasal cavity malignant melanoma(MM)tissues and analyze their relationships with clinicopathological characteristics and patient prognosis.Methods A total of 20 patients with pathologically confirmed nasal cavity MM(malignant group)and 20 with nasal inverted papilloma(benign group)were enrolled from the Second Affiliated Hospital of Hebei North University between October 2018 and October 2022.The protein and gene expression levels of P62 and PTEN were detected using immunohistochemical SP and real-time quantitative PCR methods,respectively.Systematic comparisons were made of P62 and PTEN expression among malignant tissues,adjacent tissues,and benign lesions.The relationships between their expression levels and clinicopathological features in the malignant group were analyzed.All malignant group patients were followed up for 1 year postoperatively to record recurrence and disease-free survival.Differences in P62 and PTEN expression between patients with and without recurrence were compared.Restricted cubic spline analysis was employed to evaluate the nonlinear relationship between gene expression and recurrence risk,while Kaplan-Meier method with Log-rank test was used to analyze differences in prognosis between patients with different P62 and PTEN expression levels.Results P62 and PTEN exhibited dynamic and opposing expression patterns across nasal tissue types.P62 levels were lowest in benign lesions(protein-positive rate:15.0%;gene expression:0.98±0.12),increased in paracancerous tissues(50.0%;1.25±0.27),and peaked in cancerous tissues(80.0%;1.83±0.25).Conversely,PTEN showed a progressive decline from the highest level in benign lesions(95.0%;1.67±0.33)to the lowest in cancerous tissues(35.0%;0.76±0.21),with all intergroup differences being statistically significant(all P<0.001).Clinicopathological analysis demonstrated that P62 expression increased with higher tumor stage(stage Ⅰ-Ⅲ:1.60±0.28 to 2.00±0.18)and lymph node metastasis(without 1.61±0.25 vs.with metastasis 1.90±0.21),while PTEN expression correspondingly decreased(Stage Ⅰ-Ⅲ:0.91±0.22 to 0.62±0.18;without 0.92±0.19 vs.with metastasis 0.71±0.19).Postoperative 1-year recurrent patients(9/20)showed higher P62 expression(2.01±0.17 vs.1.67±0.19)and lower PTEN expression(0.60±0.18 vs.0.89±0.14)in cancerous tissues,with recurrence risk demonstrating a nonlinear dose-response relationship with P62(≥1.760)and PTEN(≤0.815)expression levels.Survival analysis indicated that P62-positive patients had significantly shorter median disease-free survival(8.0 months vs.12.0 months),while PTEN-positive patients showed better prognosis(12.0 months vs.7.0 months),with all differences being statistically significant(P<0.05).Conclusion Elevated P62 and reduced PTEN expression were significantly correlated with malignant progression and postoperative recurrence in nasal MM.These two markers show promise as potential molecular biomarkers for assessing tumor biological behavior and prognosis,demonstrating considerable clinical translational value.
Keywords：Malignant melanomaCavidade nasalSequestosome 1Phosphatase and tensin homolog deleted on chromosome 10Clinical significance
Publication Date：2025-10-28
Online Publishing Date：2025-12-03(First online date of this platform, not the publication date of the document)
Pages：7( 999-1005 )